Ultra-HPLC鈥揗Sn (Poly)phenolic Profiling and Chemometric Analysis of Juices from Ancient Punica granatum L. Cultivars: A Nontargeted Approach
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- 作者：Luca Calani ; Deborah Begh猫 ; Pedro Mena ; Daniele Del Rio ; Renato Bruni ; Andrea Fabbri ; Chiara Dall鈥橝sta ; Gianni Galaverna
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2013
- 出版时间：June 12, 2013
- 年：2013
- 卷：61
- 期：23
- 页码：5600-5609
- 全文大小：377K
- 年卷期：v.61,no.23(June 12, 2013)
- ISSN：1520-5118
文摘
This study deals with the qualitative characterization of the phenolic profile of pomegranate juices obtained from ancient accessions. Composition data, together with genetic, morphological, and agronomical parameters, may lead to a full characterization of such germplasm, with the aim of its retrieval and biodiversity valorization. Environmental adaptation, indeed, may contribute to an enrichment of the phenolic content in pomegranate, with important effects on its nutritional properties. More than 65 punicalagins, ellagic acid derivatives, flavonoids, anthocyanins, and phenylpropanoids were simultaneously detected from four centuries old Punica granatum L. ecotypes from northern Italy and compared with those of P. granatum cv. Dente di Cavallo, a widely cultivated Italian cultivar, using a simple ultra-HPLC (uHPLC) separation and MSn linear ion trap mass spectrometric characterization. Fingerprinting phytochemical discrimination of the accessions was obtained by chemometric analysis despite their limited geographical distribution, confirming the great intraspecific variability in pomegranate secondary metabolism. The combined recourse to uHPLC鈥揗Sn qualitative fingerprinting and multivariate analysis may represent a useful tool for the discrimination and selection of pomegranate germplasm with specific properties related to polyphenolic content.