Determination of Methylglyoxal in Ruminal Fluid by High-Performance Liquid Chromatography Using Fluorometric Detection
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- 作者：Shanna L. Lodge-Ivey ; Tammy May ; Mark K. Petersen ; and James R. Strickland
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2004
- 出版时间：November 17, 2004
- 年：2004
- 卷：52
- 期：23
- 页码：6875 - 6878
- 全文大小：48K
- 年卷期：v.52,no.23(November 17, 2004)
- ISSN：1520-5118
文摘
There is no reported method for the quantification of methylglyoxal in ruminal fluid. The methodreported here is based on the conversion of methylglyoxal to 6-methylpterin, followed by quantificationof the resulting pteridinic compound by fluormetric detection using liquid chromatography. Ruminalfluid was collected and preserved with 1 M HCl at -20 
C. Cation exchange prior to derivatizationwas used to eliminate possible interfering peaks. The detection limit of 0.125 
g/mL was calculated.The recoveries were >80%, and the coefficients of variation were <15%. This method has provento be rugged and accurate for the detection of methylglyoxal concentration in ruminal fluid collectedfrom cows fed diets deficient in degradable intake protein as a marker. Methylglyoxal is produced byruminal bacteria in response to low nitrogen levels in the rumen. The ruminal methylglyoxalconcentration has the potential to be a useful marker to assess ruminal nitrogen status to aid inmore accurate diet formulation.Keywords: Methylglyoxal; HPLC; ruminants; nitrogen, carbohydrate
C. Cation exchange prior to derivatizationwas used to eliminate possible interfering peaks. The detection limit of 0.125 g/mL was calculated.The recoveries were >80%, and the coefficients of variation were <15%. This method has provento be rugged and accurate for the detection of methylglyoxal concentration in ruminal fluid collectedfrom cows fed diets deficient in degradable intake protein as a marker. Methylglyoxal is produced byruminal bacteria in response to low nitrogen levels in the rumen. The ruminal methylglyoxalconcentration has the potential to be a useful marker to assess ruminal nitrogen status to aid inmore accurate diet formulation.Keywords: Methylglyoxal; HPLC; ruminants; nitrogen, carbohydrate