Highly Sensitive and Selective Aptamer-Based Fluorescence Detection of a Malarial Biomarker Using Single-Layer MoS2 Nanosheets

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• 作者：Kenry ; Alisha Geldert ; Xiao Zhang ; Hua Zhang ; Chwee Teck Lim
• 刊名：ACS Sensor
• 出版年：2016
• 出版时间：November 23, 2016
• 年：2016
• 卷：1
• 期：11
• 页码：1315-1321
• 全文大小：578K
• ISSN：2379-3694

文摘

We demonstrate a highly selective and sensitive aptamer-based “capture-release” fluorescence detection of a malarial biomarker, i.e., Plasmodium lactose dehydrogenase (pLDH) protein, using single-layer two-dimensional MoS₂ nanosheets. The detection of the target pLDH protein utilizing the aptamer-nanosheet sensing platform is rapid and can be easily completed within 10 min. In addition, the developed biomolecular sensor is capable of detecting the target malarial biomarker in homogeneous protein solutions as well as in heterogeneous mixtures of proteins. We anticipate that the ultrathin MoS₂ nanosheet-based biosensor will facilitate the further development of biomolecular nanosensors for the detection of a wide range of biomarkers for malaria and other diseases.