Quantitative Matrix-Assisted Laser Desorption Ionization−Fourier Transform Ion Cyclotron Resonance (MALDI−FT-ICR) Peptide Profiling and Identification of Multiple-Sclerosis-Related Protein

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• 作者：Marcel P. Stoop^† ; ; Lennard J. Dekker^† ; ; Mark K. Titulaer^† ; ; Robert-Jan A. N. Lamers^† ; ; Peter C. Burgers^† ; ; Peter A. E. S
• 刊名：Journal of Proteome Research
• 出版年：2009
• 出版时间：March 6, 2009
• 年：2009
• 卷：8
• 期：3
• 页码：1404-1414
• 全文大小：251K
• 年卷期：v.8,no.3(March 6, 2009)
• ISSN：1535-3907

文摘

We introduce a matrix-assisted laser desorption ionization−Fourier transform ion cyclotron resonance (MALDI−FT-ICR) method for quantitative peptide profiling, using peak height as a measure for abundance. Relative standard deviations in peak height of peptides spiked over 3 orders of magnitude in concentration were below 10% and allowed for accurate comparisons between multiple sclerosis and controls. Application on a set of 163 cerebrospinal fluid (CSF) samples showed significantly differential abundant peptides, which were subsequently identified into proteins (e.g., chromogranin A, clusterin, and complement C3).