Essential oil of
Haplopappus greenei A. Gray was obtained by hydrodistillation of aerial parts, whichwere subsequently analyzed by gas chromatography and gas chromatography-mass spectrometry.Major components were identified as carvacrol (8.7%),
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-pinene (7.6%),
trans-pinocarveol (6.2%),and caryophyllene oxide (5.8%), respectively. In total, 104 components representing 84.9% of theinvestigated essential oil were characterized. Furthermore, the essential oil was evaluated forantimalarial, antimicrobial, and antifungal activities. However, only antifungal activity was observedagainst the strawberry anthracnose-causing fungal plant pathogens
Colletotrichum acutatum,
Colletotrichum fragariae, and
Colletotrichum gloeosporioides using the direct overlay bioautographyassay. Major essential oil components were also evaluated for antifungal activity; the carvacrolstandard demonstrated nonselective activity against the three
Colletotrichum species and the othercompounds were inactive.Keywords:
Haplopappus greenei; Asteraceae; essential oil; GC-MS; carvacrol;
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-pinene; strawberrypathogenic fungi; biological activity; antifungal activity