Control of Antigen Presentation with a Photoreleasable Agonist Peptide

详细信息    查看全文

• 作者：Andrew L. DeMond ; Toby Starr ; Michael L. Dustin ; Jay T. Groves
• 刊名：Journal of the American Chemical Society
• 出版年：2006
• 出版时间：December 6, 2006
• 年：2006
• 卷：128
• 期：48
• 页码：15354 - 15355
• 全文大小：197K
• 年卷期：v.128,no.48(December 6, 2006)
• ISSN：1520-5126

文摘

The immunological synapse is a specialized intercellular junction between a T cell and a target cell that orchestrates the engagement of receptors and ligands in space and time as a means of regulating function. Here we introduce a reagent for controlling the spatial and temporal presentation of natural antigen to T cells. Moth cytochrome c (88-103) peptide (MCC), an agonist to the murine T cell receptor AND when presented in the context of H2 IE^k major histocompatibility complex (IE^k), was synthesized with the side-chain amine of Lys99 conjugated to a photosensitive protecting group, 6-nitroveratryloxycarbonyl (NVOC). Cells plated on supported bilayers displaying mobile intercellular adhesion molecule-1 (ICAM-1) and NVOC-MCC loaded IE^k did not form immunological synapses and exhibited low intracellular calcium levels, similar to cells presented with self-peptide. Irradiation with UV light was sufficient to restore agonist activity in situ.