Antineoplastic Agents. 560. Isolation and Structure of Kitastatin 1 from an Alaskan Kitasatospora sp.1
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- 作者：George R. Pettit ; Rui Tan ; Robin K. Pettit ; Thomas H. Smith ; Song Feng ; Dennis L. Doubek ; Linda Richert ; John Hamblin ; Christine Weber ; Jean-Charles Chapuis
- 刊名：Journal of Natural Products
- 出版年：2007
- 出版时间：July 2007
- 年：2007
- 卷：70
- 期：7
- 页码：1069 - 1072
- 全文大小：53K
- 年卷期：v.70,no.7(July 2007)
- ISSN：1520-6025
文摘
By utilizing a bioassay-guided separation (P388 lymphocytic leukemia and a panel of human cancer cell lines) offermentation broths from a Kitasatospora sp. collected from a tundra soil sample taken at the shore of the Beaufort Sea,we have isolated three powerful (GI50 to 0.0006 
g/mL) cancer cell growth inhibitors (1-3) and determined their structuresto be closely related cyclodepsipeptides. From 380 L fermentations of Kitasatospora sp. were obtained 2.6 mg of a newcyclodepsipeptide designated kitastatin 1 (3), accompanied by the previously known respirantin (1, 10.8 mg) and itsvaleryl homologue (2, 4.8 mg). The structures were determined by employment of a series of high-resolution mass and2D NMR spectroscopic analyses. The stereochemical assignments and overall structures were confirmed by subsequenttotal synthesis of depsipeptide 1, as reported in the accompanying contribution.
g/mL) cancer cell growth inhibitors (1-3) and determined their structuresto be closely related cyclodepsipeptides. From 380 L fermentations of Kitasatospora sp. were obtained 2.6 mg of a newcyclodepsipeptide designated kitastatin 1 (3), accompanied by the previously known respirantin (1, 10.8 mg) and itsvaleryl homologue (2, 4.8 mg). The structures were determined by employment of a series of high-resolution mass and2D NMR spectroscopic analyses. The stereochemical assignments and overall structures were confirmed by subsequenttotal synthesis of depsipeptide 1, as reported in the accompanying contribution.