The Smart Petri Dish: A Nanostructured Photonic Crystal for Real-Time Monitoring of Living Cells

详细信息    查看全文

• 作者：Michael P. Schwartz ; Austin M. Derfus ; Sara D. Alvarez ; Sangeeta N. Bhatia ; Michael J. Sailor
• 刊名：Langmuir
• 出版年：2006
• 出版时间：August 1, 2006
• 年：2006
• 卷：22
• 期：16
• 页码：7084 - 7090
• 全文大小：655K
• 年卷期：v.22,no.16(August 1, 2006)
• ISSN：1520-5827

文摘

The intensity of light scattered from a porous Si photonic crystal is used to monitor physiological changes in primaryrat hepatocytes. The cells are seeded on the surface of a porous Si photonic crystal that has been filled with polystyreneand treated with an O₂ plasma. Light resonant with the photonic crystal is scattered by the cell layer and detectedas an optical peak with a charge-coupled-device spectrometer. It is demonstrated that exposure of hepatocytes to thetoxins cadmium chloride or acetaminophen leads to morphology changes that cause a measurable increase in scatteredintensity. The increase in signal occurs before traditional assays are able to detect a decrease in viability, demonstratingthe potential of the technique as a complementary tool for cell viability studies. The scattering method presented hereis noninvasive and can be performed in real time, representing a significant advantage compared to other techniquesfor in vitro monitoring of cell morphology.