Individual Platelet Adhesion Assay: Measuring Platelet Function and Antiplatelet Therapies in Whole Blood via Digital Quantification of Cell Adhesion

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• 作者：Ana Lopez-Alonso ; Bincy Jose ; Martin Somers ; Karl Egan ; David P. Foley ; Antonio J. Ricco ; Sofia Ramstr枚m ; Lourdes Basabe-Desmonts ; Dermot Kenny
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：July 2, 2013
• 年：2013
• 卷：85
• 期：13
• 页码：6497-6504
• 全文大小：425K
• 年卷期：v.85,no.13(July 2, 2013)
• ISSN：1520-6882

文摘

Widespread monitoring of platelet function and the effect of antiplatelet drugs will improve outcomes in cardiovascular patients, but platelet function testing is not routine in clinical practice. We report a rapid, accurate methodology to quantify platelet-protein interactions: a microarray of contact-printed 6-渭m fibrinogen dots on a transparent substrate binds platelets from whole blood, one platelet per dot. The fractional occupancy of an array of fibrinogen dots after a predefined incubation time quantitatively assays platelet adhesion to the protein matrix. We demonstrate this technique by measurement of platelet adhesion to fibrinogen as a means to quantify the effect of the P2Y₁₂ and 伪IIb尾3 receptor inhibitors cangrelor and abciximab, respectively, both in vitro鈥攂y incubating the drug with a freshly drawn blood sample鈥攁nd in blood from patients treated with antiplatelet agents. The effects of single- and dual-antiplatelet therapy are also assessed. Results from this platelet-binding assay are well correlated with standard techniques including flow cytometry and light transmission aggregometry. This assay technology, readily integrated with microfluidic platforms, is generally applicable to the assay of cell-protein interactions and promises more effective, rapid assay of drug effects in cardiovascular disease patients.