Proteomics Analysis of Rat Brain Protein Modulations by Grape Seed Extract

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• 作者：Jessy Deshane ; Lisa Chaves ; Kiran Varma Sarikonda ; Scott Isbell ; Landon Wilson ; Marion Kirk ; Clinton Grubbs ; Stephen Barnes ; Sreelatha Meleth ; and Helen Kim
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2004
• 出版时间：December 29, 2004
• 年：2004
• 卷：52
• 期：26
• 页码：7872 - 7883
• 全文大小：473K
• 年卷期：v.52,no.26(December 29, 2004)
• ISSN：1520-5118

文摘

Dietary supplements such as grape seed extract (GSE) enriched in proanthocyanidins (PA) (oligomericpolyphenols) have been suggested to have multiple health benefits, due to antioxidant and otherbeneficial activities of the PA. However, a systematic analysis of the molecular basis of these benefitshas not been demonstrated. Because the brain is vulnerable to age-related oxidative damage andother insults including inflammation, it was hypothesized that rats ingesting GSE would experiencechanges in expression or modifications of specific brain proteins that might protect against pathologicevents. Normal adult female rats were fed diets supplemented with 5% GSE for 6 weeks. Proteomicsanalysis (2D electrophoresis and mass spectrometry) of brain homogenates from these animalsidentified 13 proteins that were altered in amount and/or charge. Because many of these changeswere quantitatively in the opposite direction from previous findings for the same proteins in eitherAlzheimer disease or mouse models of neurodegeneration, the data suggest that these identifiedproteins may mediate the neuroprotective actions of GSE. This is the first identification and quantitationof specific proteins in mammalian tissues modulated by a dietary supplement, as well as the first todemonstrate links of such proteins with any disease.Keywords: Grape polyphenols; dietary supplement; 2D electrophoresis; mass spectrometry; dementia