文摘
In this study we investigated some biological properties of flavonoids recovered in the aqueous (AqE)and ether (EtE) extracts from four Italian multifloral honeys. In particular, a cell-free assay wasemployed to detect direct reduction of ferricyanide, whereas an assay using intact human erythrocyteswas used to measure the ability to donate electrons to a trans-plasma membrane oxidoreductase. Itwas found that the AqE displays greater "in vitro" ferricyanide-reducing activity than the EtE but,unlike the latter, is virtually ineffective in the cell-based assay. Uptake studies employing high-performance liquid chromatography/mass spectrometry (HPLC/MS) showed that the different resultswere explained by the inability of AqE components to cross the erythrocyte plasma membrane andby the excellent uptake of EtE flavonoids, which, once within the cell, donate electrons to the membraneoxidoreductase to efficiently reduce extracellular oxidants. The latter property appears to depend onthe content of ether-soluble flavonoids in the starting honeys.