Photoinitiated Coupling of Unmodified Monosaccharides to Iron Oxide Nanoparticles for Sensing Proteins and Bacteria

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• 作者：Li-Hong Liu ; Herv Dietsch ; Peter Schurtenberger ; Mingdi Yan
• 刊名：Bioconjugate Chemistry
• 出版年：2009
• 出版时间：July 15, 2009
• 年：2009
• 卷：20
• 期：7
• 页码：1349-1355
• 全文大小：230K
• 年卷期：v.20,no.7(July 15, 2009)
• ISSN：1520-4812

文摘

We report a versatile approach for the immobilization of unmodified monosaccharides onto iron oxide nanoparticles. Covalent coupling of the carbohydrate onto iron oxide nanoparticle surfaces was accomplished by the CH insertion reaction of photochemically activated phosphate-functionalized perfluorophenylazides (PFPAs), and the resulting glyconanoparticles were characterized by IR, TGA, and TEM. The surface-bound d-mannose showed the recognition ability toward Concanavalin A and Escherichia coli strain ORN178 that possesses mannose-specific receptor sites. Owing to the simplicity and versatility of the technique, together with the magnetic property of iron oxide nanoparticles, the methodology developed in this study serves as a general approach for the preparation of magnetic glyconanoparticles to be used in clinical diagnosis, sensing, and decontamination.