A Metabonomic Investigation on the Biochemical Perturbation in Liver Failure Patients Caused by Hepatitis B Virus

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• 作者：Ke Yu ; Guoping Sheng ; Jifang Sheng ; Yuemei Chen ; Wei Xu ; Xiaoli Liu ; Hongcui Cao ; Haibin Qu ; Yiyu Cheng ; Lanjuan Li
• 刊名：Journal of Proteome Research
• 出版年：2007
• 出版时间：July 2007
• 年：2007
• 卷：6
• 期：7
• 页码：2413 - 2419
• 全文大小：295K
• 年卷期：v.6,no.7(July 2007)
• ISSN：1535-3907

文摘

A metabonomic study was performed to investigate the biochemical perturbation of the serum samplesfrom liver failure patients induced by hepatitis B virus (HBV; n = 24) and control normal subjects (n =23). The serum metabonome was detected using gas chromatography-mass spectrometry (GC-MS)technique integrated with a commercial mass spectral library for the peak identification. After peakdeconvolution, identification, and matching, the acquired GC-MS data were normalized and processedby principal component analysis (PCA) and canonical discriminant analysis (CDA). Specific changes inthe metabolic composition of serum samples from patients including amino acids (AAs) and glucosewere shown in GC-MS total ion current (TIC) chromatograms. The distinctive biochemical differencebetween the healthy subjects and liver failure patients was displayed by the pattern recognition methods.We also found that the liver failure patients with different degree of severity categorized as MELD(model for end-stage of liver diseases) could be clearly classified by the corresponding metabonomicdata. In comparison, the current routine clinical indices cannot characterize the global phenotyping ofliver failure. The result demonstrated that the GC-MS technique is an alternative tool for thecharacterization of the metabolic perturbation and the metabonomic study promises to provide anintegrative criterion to evaluate the severity and the prognosis of liver diseases.