Protein/Polysaccharide Interactions and Their Impact on Haze Formation in White Wines

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• 作者：Marie Dufrechou ; Thierry Doco ; C茅line Poncet-Legrand ; Fran莽ois-Xavier Sauvage ; Aude Vernhet
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：November 18, 2015
• 年：2015
• 卷：63
• 期：45
• 页码：10042-10053
• 全文大小：640K
• ISSN：1520-5118

文摘

Proteins in white wines may aggregate and form hazes at room temperature. This was previously shown to be related to pH-induced conformational changes and to occur for pH <3.5. The aim of the present work was to study the impact of wine polysaccharides on pH-induced haze formation by proteins but also the consequences of their interactions with these proteins on the colloidal stability of white wines. To this end, model systems and purified global pools of wine proteins and polysaccharides were used first. Kinetics of aggregation, proteins involved, and turbidities related to final hazes were monitored. To further identify the impact of each polysaccharide, fractions purified to homogeneity were used in a second phase. These included two neutral (mannoprotein and arabinogalactan) and two negatively charged (rhamnogalacturonan II dimer (RG-II) and arabinogalactan) polysaccharides. The impact of major wine polysaccharides on wine protein aggregation at room temperature was clearly less marked than those of the pH and the ionic strength. Polysaccharides modulated the aggregation kinetics and final haziness, indicating that they interfere with the aggregation process, but could not prevent it.