Mass Production of Rubusoside Using a Novel Stevioside-Specific 尾-Glucosidase from Aspergillus aculeatus
详细信息 查看全文
- 作者：Jin-A Ko ; Young-Min Kim ; Young Bae Ryu ; Hyung Jae Jeong ; Tae-Soon Park ; Su-Jin Park ; Young-Jung Wee ; Joong-Su Kim ; Doman Kim ; Woo Song Lee
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2012
- 出版时间：June 20, 2012
- 年：2012
- 卷：60
- 期：24
- 页码：6210-6216
- 全文大小：359K
- 年卷期：v.60,no.24(June 20, 2012)
- ISSN：1520-5118
文摘
Rubusoside (R) is a natural sweetener and a solubilizing agent with antiangiogenic and antiallergic properties. However, currently, its production is quite expensive, and therefore, we have investigated nine commercially available glycosidases to optimize an economically viable R-production method. A stevioside (ST)-specific 尾-glucosidase (SSGase) was selected and purified 7-fold from Aspergillus aculeatus Viscozyme L by a two-step column chromatography procedure. The 79 kDa protein was stable from pH 3.0 to pH 7.0 at 50鈥?0 掳C. Hydrolysis of ST by SSGase produced R and steviol monoglucosyl ester as determined by 1H and 13C nuclear magnetic resonance (NMR). Importantly, SSGase showed higher activity toward ST than other 尾-linked glucobioses. The optimal conditions for R production were 280 mM ST and 16.6 渭L of SSGase at pH 5.1 and 63 掳C. This is the first discussion detailing the production of R by enzymatic hydrolysis of ST and is useful for the food additive and pharmaceutical industries.