One-Pot Synthesis of Iron Oxide Nanoparticles with Functional Silane Shells: A Versatile General Precursor for Conjugations and Biomedical Applications

详细信息    查看全文

• 作者：Vinith Yathindranath ; Zhizhi Sun ; Matthew Worden ; Lynda J. Donald ; James A. Thliveris ; Donald W. Miller ; Torsten Hegmann
• 刊名：Langmuir
• 出版年：2013
• 出版时间：August 27, 2013
• 年：2013
• 卷：29
• 期：34
• 页码：10850-10858
• 全文大小：591K
• 年卷期：v.29,no.34(August 27, 2013)
• ISSN：1520-5827

文摘

Iron oxide nanoparticles (IONPs) and their surface modifications with therapeutic or diagnostic (theranostic, TN) agents are of great interest. Here we present a novel one-pot synthesis of a versatile general TN precursor (aminosilane-coated IONPs [IONP-Sil(NH₂)]) with surface amine groups. Surface functional group conversion to carboxylic acid was accomplished by conjugating poly(ethylene glycol) diacid to IONP-Sil(NH₂). The NPs were characterized using powder X-ray diffraction (XRD), transmission electron microscopy (TEM), high-resolution TEM, selected area electron diffraction (SAED), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared (FT-IR) spectroscopy. Biocompatibility and cell uptake profile of the nanoparticles were evaluated in-vitro using cultured liver cells (HepG2). Oleylamine (hydrophobic) and bovine serum albumin (BSA) as model drugs were attached to IONP-Sil-PEG(COOH). The ability of IONP-Sil(NH₂) to bind small interfering RNA (siRNA) is also shown.