Direct Analysis of Thin-Layer Chromatography Separations of Petroleum Samples by Laser Desorption Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Imaging

详细信息    查看全文

• 作者：Donald F. Smith ; Amy M. McKenna ; Yuri E. Corilo ; Ryan P. Rodgers ; Alan G. Marshall ; Ron M. A. Heeren
• 刊名：Energy & Fuels
• 出版年：2014
• 出版时间：October 16, 2014
• 年：2014
• 卷：28
• 期：10
• 页码：6284-6288
• 全文大小：331K
• ISSN：1520-5029

文摘

We present an analytical method for direct analysis of thin-layer chromatography (TLC) separations of petroleum samples by laser desorption ionization (LDI) coupled to Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) imaging. LDI of TLC plates selectively ionizes condensed aromatic hydrocarbons and facilitates two-dimensional imaging of TLC-separated petroleum compounds. Molecular-level characterization available only with ultrahigh-resolution FT-ICR MS provides elemental composition assignment and surpasses conventional TLC readout-based flame ionization detection. Resolution and assignment of migrated molecules targeted by LDI combined with FT-ICR MS provides elemental composition assignment and, therefore, chemical information, i.e., heteroatom class, aromaticity (double bond equivalents), and carbon number. Here, three petroleum samples (a field deposit, a crude oil, and a tar ball) were TLC-separated by their solubility in n-heptane and imaged by LDI FT-ICR MS.