Virus鈥揚olymer Hybrid Nanowires Tailored to Detect Prostate-Specific Membrane Antigen

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• 作者：Jessica A. Arter ; Juan E. Diaz ; Keith C. Donavan ; Tom Yuan ; Reginald M. Penner ; Gregory A. Weiss
• 刊名：Analytical Chemistry
• 出版年：2012
• 出版时间：March 20, 2012
• 年：2012
• 卷：84
• 期：6
• 页码：2776-2783
• 全文大小：475K
• 年卷期：v.84,no.6(March 20, 2012)
• ISSN：1520-6882

文摘

We demonstrate the de novo fabrication of a biosensor, based upon virus-containing poly(3,4-ethylene-dioxythiophene) (PEDOT) nanowires, that detects prostate-specific membrane antigen (PSMA). This development process occurs in three phases: (1) isolation of a M13 virus with a displayed polypeptide receptor, from a library of 鈮?0¹¹ phage-displayed peptides, which binds PSMA with high affinity and selectivity, (2) microfabrication of PEDOT nanowires that entrain these virus particles using the lithographically patterned nanowire electrodeposition (LPNE) method, and (3) electrical detection of the PSMA in high ionic strength (150 mM salt) media, including synthetic urine, using an array of virus鈥揚EDOT nanowires with the electrical resistance of these nanowires for transduction. The electrical resistance of an array of these nanowires increases linearly with the PSMA concentration from 20 to 120 nM in high ionic strength phosphate-buffered fluoride (PBF) buffer, yielding a limit of detection (LOD) for PSMA of 56 nM.