Nanoneedle Transistor-Based Sensors for the Selective Detection of Intracellular Calcium Ions

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• 作者：Donghee Son ; Sung Young Park ; Byeongju Kim ; Jun Tae Koh ; Tae Hyun Kim ; Sangmin An ; Doyoung Jang ; Gyu Tae Kim ; Wonho Jhe ; Seunghun Hong
• 刊名：ACS Nano
• 出版年：2011
• 出版时间：May 24, 2011
• 年：2011
• 卷：5
• 期：5
• 页码：3888-3895
• 全文大小：945K
• 年卷期：0
• ISSN：1936-086X

文摘

We developed a nanoneedle transistor-based sensor (NTS) for the selective detection of calcium ions inside a living cell. In this work, a single-walled carbon nanotube-based field effect transistor (swCNT-FET) was first fabricated at the end of a glass nanopipette and functionalized with Fluo-4-AM probe dye. The selective binding of calcium ions onto the dye molecules altered the charge state of the dye molecules, resulting in the change of the source-drain current of the swCNT-FET as well as the fluorescence intensity from the dye. We demonstrated the electrical and fluorescence detection of the concentration change of intracellular calcium ions inside a HeLa cell using the NTS.