Use of Ionic Liquid To Significantly Improve Asymmetric Reduction of Ethyl Acetoacetate Catalyzed by Acetobacter sp. CCTCC M209061 Cells

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• 作者：Xiao-Ting Wang ; Dong-Mei Yue ; Min-Hua Zong ; Wen-Yong Lou
• 刊名：Industrial & Engineering Chemistry Research
• 出版年：2013
• 出版时间：September 4, 2013
• 年：2013
• 卷：52
• 期：35
• 页码：12550-12558
• 全文大小：532K
• 年卷期：v.52,no.35(September 4, 2013)
• ISSN：1520-5045

文摘

Biocatalytic reduction of ethyl acetoacetate (EAA) to ethyl (R)-3-hydroxybutyrate [(R)-EHB] with Acetobacter sp. CCTCC M209061 cells was successfully conducted in ionic liquid (IL)-based biphasic systems. Several water-immiscible ILs were used to construct biphasic systems. The best IL investigated was 1-butyl-3-methylimidazolium hexafluorophosphate (C₄mim路PF₆), which also had good biocompatibility. Several influential variables were examined. The optimum parameters were as follows: volume ratio of buffer to C₄mim路PF₆, 1/2 (v/v); substrate concentration, 55 mmol/L; buffer pH, 5.5; cosubstrate concentration, 80 mmol/L; reaction temperature, 35 掳C; and shaking speed, 220 rpm. Under these optimal conditions, the initial reaction rate, the yield, and the product e.e. were 0.39 mmol/(L min), 90.8%, and >99%, respectively, which were much better than results reported previously. This efficient whole-cell biocatalytic process was feasible on a 450-mL preparative scale, and the immobilized cells showed excellent operational stability and could be reused for at least 10 batches.