Efficient Synthesis of a Chiral Precursor for Angiotensin-Converting Enzyme (ACE) Inhibitors in High Space-Time Yield by a New Reductase without External Cofactors
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- 作者：Nai-Dong Shen ; Yan Ni ; Hong-Min Ma ; Li-Juan Wang ; Chun-Xiu Li ; Gao-Wei Zheng ; Jie Zhang ; Jian-He Xu
- 刊名：Organic Letters
- 出版年：2012
- 出版时间：April 20, 2012
- 年：2012
- 卷：14
- 期：8
- 页码：1982-1985
- 全文大小：212K
- 年卷期：v.14,no.8(April 20, 2012)
- ISSN：1523-7052
文摘
A new reductase, CgKR2, with the ability to reduce ethyl 2-oxo-4-phenylbutyrate (OPBE) to ethyl (R)-2-hydroxy-4-phenylbutyrate ((R)-HPBE), an important chiral precursor for angiotensin-converting enzyme (ACE) inhibitors, was discovered. For the first time, (R)-HPBE with >99% ee was produced via bioreduction of OPBE at 1 M without external addition of cofactors. The space-time yield (700 g路L鈥?路d鈥?) was 27 times higher than the highest record.