Layer-by-Layer Assembled Polypeptide Capsules for Platinum-Based Pro-Drug Delivery

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• 作者：Dongfang Zhou ; Haihua Xiao ; Fanbo Meng ; Shaoyang Zhou ; Jinshan Guo ; Xiaoyuan Li ; Xiabin Jing ; Yubin Huang
• 刊名：Bioconjugate Chemistry
• 出版年：2012
• 出版时间：December 19, 2012
• 年：2012
• 卷：23
• 期：12
• 页码：2335-2343
• 全文大小：392K
• 年卷期：v.23,no.12(December 19, 2012)
• ISSN：1520-4812

文摘

Platinum(IV), a pro-drug of platinum(II), was conjugated to poly(l-lysine) (PLL), and then assembled with poly(glutamic acid) (PGA) through a layer-by-layer (LbL) approach on colloidal silica templates. After removal of the templates, biodegradable PGA/PLL-Pt(IV) multilayer capsules (diameter = 0.5 渭m) with 10 渭g of platinum incorporated into each bilayer were obtained. Under acidic and/or reductive conditions, the amount and rate of platinum released from the capsules were increased, which are desirable traits for platinum-based anticancer drug delivery systems. Furthermore, in vitro evaluation showed that the PGA/PLL-Pt(IV) multilayer microcapsules displayed higher cytotoxicity (IC_50Pt = 3.5 渭g/mL) against colon cancer cells CT-26 than that of free cisplatin (IC_50Pt = 8.6 渭g/mL). This enhanced cytotoxicity was attributed to the effective internalization of the capsules by the cancer cells, which was observed by confocal laser scanning microscopy (CLSM) imaging.