Fast, Efficient, and Gentle Transfection of Human Adherent Cells in Suspension
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- 作者：Pranav Agrawal ; Nilesh P. Ingle ; William S. Boyle ; Emily Ward ; Jakub Tolar ; Kevin D. Dorfman ; Theresa M. Reineke
- 刊名：ACS Applied Materials & Interfaces
- 出版年：2016
- 出版时间：April 13, 2016
- 年：2016
- 卷：8
- 期：14
- 页码：8870-8874
- 全文大小：383K
- 年卷期：0
- ISSN：1944-8252
文摘
We demonstrate a highly efficient method for gene delivery into clinically relevant human cell types, such as induced pluripotent stem cells (iPSCs) and fibroblasts, reducing the protocol time by one full day. To preserve cell physiology during gene transfer, we designed a microfluidic strategy, which facilitates significant gene delivery in a short transfection time (<1 min) for several human cell types. This fast, optimized and generally applicable cell transfection method can be used for rapid screening of different delivery systems and has significant potential for high-throughput cell therapy applications.