Topographical Variation in Metabolic Signatures of Human Gastrointestinal Biopsies Revealed by High-Resolution Magic-Angle Spinning 1H NMR Spectroscopy
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- 作者：Yulan Wang ; Elaine Holmes ; Elena M. Comelli ; Grigorios Fotopoulos ; Gian Dorta ; Huiru Tang ; Mattias J Rantalainen ; John C Lindon ; Irè ; ne E Corthé ; sy-Theulaz ; Laurent B. Fay ; Sunil Kochhar ; J
- 刊名：Journal of Proteome Research
- 出版年：2007
- 出版时间：October 2007
- 年：2007
- 卷：6
- 期：10
- 页码：3944 - 3951
- 全文大小：245K
- 年卷期：v.6,no.10(October 2007)
- ISSN：1535-3907
文摘
Individual and topographical variation in the metabolic profiles of multiple human gastrointestinal tract(GIT) biopsies have been characterized using high-resolution magic-angle spinning (HRMAS) 1H NMRspectroscopy and pattern recognition. Samples from antrum, duodenum, jejunum, ileum, and transversecolon were obtained from 8 male and 8 female participants. Each gut region generated a highlycharacteristic metabolic profile consistent with the varying structural and functional properties of thetissue at different longitudinal levels of the gut. The antral (stomach) mucosa contained higher levelsof choline, glycogen, phosphorylethanolamine, and taurine than other gut regions. The spatially closeregions of the duodenum and jejunum were equivalent in terms of their gross biochemical compositionwith high levels of choline, glutathione, glycerophosphocholine (GPC), and lipids relative to other gutregions. The ileal mucosa showed poor discrimination from the duodenum and jejunum tissues andgenerated strong amino acids signatures but had relative low GPC signals. The colon (large intestine)was high in acetate, glutamate, inositols, and lactate and low in creatine, GPC, and taurine comparedto the small intestine. These longitudinal metabolic variations in the human GIT could be attributed tofunctional variations in energy metabolism, osmoregulation, gut microbial activity, and oxidativeprotection. This work indicates that 1H HRMAS NMR studies may be of value in analyzing local metabolicvariation due to pathological processes in gut biopsies.