Dynamic Kinetic Capillary Isoelectric Focusing: A Powerful Tool for Studying Protein-DNA Interactions

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• 作者：Zhen Liu ; Andrei P. Drabovich ; Sergey N. Krylov ; Janusz Pawliszyn
• 刊名：Analytical Chemistry
• 出版年：2007
• 出版时间：February 1, 2007
• 年：2007
• 卷：79
• 期：3
• 页码：1097 - 1100
• 全文大小：106K
• 年卷期：v.79,no.3(February 1, 2007)
• ISSN：1520-6882

文摘

A new method called dynamic kinetic capillary isoelectricfocusing (DK-CIEF) is presented for the study of protein-DNA interactions. The method is based on CIEF withlaser-induced fluorescence-whole column imaging detection in which protein-DNA complexes are separated withspatial resolution while dissociations of the complexes aredynamically monitored using a CCD camera with temporalresolution. This method allows for the discrimination ofdifferent complexes and the measurement of the individual dissociation rate constants.