Optimized Formulation of a Thermostable Spray-Dried Virus-Like Particle Vaccine against Human Papillomavirus

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• 作者：Sugandha Saboo ; Ebenezer Tumban ; Julianne Peabody ; Denis Wafula ; David S. Peabody ; Bryce Chackerian ; Pavan Muttil
• 刊名：Molecular Pharmaceutics
• 出版年：2016
• 出版时间：May 2, 2016
• 年：2016
• 卷：13
• 期：5
• 页码：1646-1655
• 全文大小：521K
• 年卷期：0
• ISSN：1543-8392

文摘

Existing vaccines against human papillomavirus (HPV) require continuous cold-chain storage. Previously, we developed a bacteriophage virus-like particle (VLP)-based vaccine for HPV infection, which elicits broadly neutralizing antibodies against diverse HPV types. Here, we formulated these VLPs into a thermostable dry powder using a multicomponent excipient system and by optimizing the spray-drying parameters using a half-factorial design approach. Dry-powder VLPs were stable after spray drying and after long-term storage at elevated temperatures. Immunization of mice with a single dose of reconstituted dry-powder VLPs that were stored at 37 °C for more than a year elicited high anti-L2 IgG antibody titers. Spray-dried thermostable, broadly protective L2 bacteriophage VLPs vaccine could be accessible to remote regions of the world (where ∼84% of cervical cancer patients reside) by eliminating the cold-chain requirement during transportation and storage.