Production of Tropane Alkaloids during De-differentiation of Scopolia parviflora Calli
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- 作者：Yong Duck Kim ; Seung Mi Kang ; Ji Yun Min ; Won Kyun Choi ; Mi Jin Jeong ; Chandrakant. S. Karigar ; Myung Suk Choi
- 刊名：Journal of Natural Products
- 出版年：2010
- 出版时间：February 26, 2010
- 年：2010
- 卷：73
- 期：2
- 页码：147-150
- 全文大小：223K
- 年卷期：v.73,no.2(February 26, 2010)
- ISSN：1520-6025
文摘
The production of tropane alkaloids during differentiation and de-differentiation of Scopolia parviflora calli was studied. Tropane alkaloid production drastically decreased during calli de-differentiation. Scopolamine (1) production decreased after 10 days of culture, whereas that of hyoscyamine (2) decreased during de-differentiation of root to calli. The production of 1 was enhanced in calli undergoing differentiation to shoot after 60 days of culture, reaching a maximum by 80 days. However, production of hyoscyamine in regenerated plants was lower. The expression level of hyoscyamine 6β-hydroxylase (H6H), a key biosynthetic enzyme for tropane alkaloids, was significantly increased in 4-week-old calli. This study suggests that the biosynthesis of tropane alkaloids is regulated inversely in de-differentiating Scopolia parviflora calli.