Microfluidic Flow-Flash: Method for Investigating Protein Dynamics

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• 作者：Michael W. Toepke ; Scott H. Brewer ; Dung M. Vu ; Kirk D. Rector ; Joel E. Morgan ; Robert B. Gennis ; Paul J. A. Kenis ; R. Brian Dyer
• 刊名：Analytical Chemistry
• 出版年：2007
• 出版时间：January 1, 2007
• 年：2007
• 卷：79
• 期：1
• 页码：122 - 128
• 全文大小：387K
• 年卷期：v.79,no.1(January 1, 2007)
• ISSN：1520-6882

文摘

We report a new method, microfluidic flow-flash, formeasuring protein reaction kinetics. The method couplesa microscope imaging detection system with a microfluidicflow cell to reduce data acquisition times and sampleconsumption. This combination allows for the simultaneous collection of spectral and temporal information. Themicrofluidic flow cell design utilizes three-dimensionalsheath flow to reduce sample dispersion and minimizesample consumption. The ability to alter the flow rates inthe microfluidic flow cells allows a variety of time scalesto be studied with submillisecond time resolution. Theimaging detection system can be coupled with severalspectroscopic probes including fluorescence and UV/visible absorbance spectroscopy. Here, we utilize themicrofluidic flow-flash method to probe the kinetics of COrecombination or O₂ binding to myoglobin after the laser-induced photolysis of CO from myoglobin by UV/visibleabsorbance spectral imaging.