Snake Venomics of Bothriechis nigroviridis Reveals Extreme Variability among Palm Pitviper Venoms: Different Evolutionary Solutions for the Same Trophic Purpose

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• 作者：Julin Fernndez ; Bruno Lomonte ; Libia Sanz ; Yamileth Angulo ; Jos Mara Gutirrez ; Juan J. Calvete
• 刊名：Journal of Proteome Research
• 出版年：2010
• 出版时间：August 6, 2010
• 年：2010
• 卷：9
• 期：8
• 页码：4234-4241
• 全文大小：226K
• 年卷期：v.9,no.8(August 6, 2010)
• ISSN：1535-3907

文摘

We report the proteomic characterization and biological activities of the venom of the black-speckled palm pitviper, Bothriechis nigroviridis, a neotropical arboreal pitviper from Costa Rica. In marked contrast to other Bothriechis species investigated, the venom of B. nigroviridis does not possess detectable Zn²⁺-dependent metalloproteinases, and is uniquely characterized by a high content of crotoxin-like PLA₂ and vasoactive peptides. These data suggest that different evolutionary solutions have evolved within the arboreal genus Bothriechis for the same trophic purpose. The venom from B. nigroviridis is devoid of hemorrhagic activity, has low edematogenic and coagulant effects, presents modest myotoxic and phospholipase A₂ activities, but has higher lethality than the venoms of other Bothriechis species. Neutralization of its lethal activity by an anti-Crotalus durissus terrificus antivenom confirmed the major role of crotoxin-like PLA₂ in B. nigroviridis venom-induced lethality.

Keywords:

Bothriechis nigroviridis; black-speckled palm pitviper; snake venom proteomics; venomics; N-terminal sequencing; mass spectrometry