Biphasic Effect of Falcarinol on CaCo-2 Cell Proliferation, DNA Damage, and Apoptosis
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- 作者：Jette F. Young ; Susan J. Duthie ; Lesley Milne ; Lars P. Christensen ; Garry G. Duthie ; Charles S. Bestwick
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2007
- 出版时间：February 7, 2007
- 年：2007
- 卷：55
- 期：3
- 页码：618 - 623
- 全文大小：246K
- 年卷期：v.55,no.3(February 7, 2007)
- ISSN：1520-5118
文摘
The polyacetylene falcarinol, isolated from carrots, has been shown to be protective against chemicallyinduced colon cancer development in rats, but the mechanisms are not fully understood. In this studyCaCo-2 cells were exposed to falcarinol (0.5-100 
M) and the effects on proliferation, DNA damage,and apoptosis investigated. Low-dose falcarinol exposure (0.5-10 M) decreased expression of theapoptosis indicator caspase-3 concomitantly with decreased basal DNA strand breakage. Cellproliferation was increased (1-10 M), whereas cellular attachment was unaffected by <10 Mfalcarinol. At concentrations above 20 
falcarinol, proliferation of CaCo-2 cells decreased and thenumber of cells expressing active caspase-3 increased simultaneously with increased cell detachment.Furthermore, DNA single-strand breakage was significantly increased at concentrations above 10M falcarinol. Thus, the effects of falcarinol on CaCo-2 cells appear to be biphasic, inducing pro-proliferative and apoptotic characteristics at low and high concentrations of falcarinol, respectively.
M) and the effects on proliferation, DNA damage,and apoptosis investigated. Low-dose falcarinol exposure (0.5-10 M) decreased expression of theapoptosis indicator caspase-3 concomitantly with decreased basal DNA strand breakage. Cellproliferation was increased (1-10 M), whereas cellular attachment was unaffected by <10 Mfalcarinol. At concentrations above 20 falcarinol, proliferation of CaCo-2 cells decreased and thenumber of cells expressing active caspase-3 increased simultaneously with increased cell detachment.Furthermore, DNA single-strand breakage was significantly increased at concentrations above 10M falcarinol. Thus, the effects of falcarinol on CaCo-2 cells appear to be biphasic, inducing pro-proliferative and apoptotic characteristics at low and high concentrations of falcarinol, respectively.