The Response of Human Colonocytes to Folate Deficiency in Vitro: Functional and Proteomic Analyses

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• 作者：Susan J. Duthie ; Yiannis Mavrommatis ; Gary Rucklidge ; Martin Reid ; Gary Duncan ; Mary P. Moyer ; Lynn P. Pirie ; Charles S. Bestwick
• 刊名：Journal of Proteome Research
• 出版年：2008
• 出版时间：August 2008
• 年：2008
• 卷：7
• 期：8
• 页码：3254-3266
• 全文大小：223K
• 年卷期：v.7,no.8(August 2008)
• ISSN：1535-3907

文摘

Low folate intake is associated with colon cancer. We combined a proteomics and biochemical approach to identify proteins and pathways affected by folate deficiency in human colonocytes. Folate differentially altered activity and expression of proteins involved in proliferation [e.g., PCNA], DNA repair [e.g., XRCC5, MSH2], apoptosis [e.g., BAG family chaperone protein, DIABLO and porin], cytoskeletal organization [e.g., actin, ezrin, elfin], and expression of proteins implicated in malignant transformation [COMT, Nit2].