Production of 7-epi-Pectenotoxin-2 Seco Acid and Assessment of Its Acute Toxicity to Mice

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• 作者：Christopher O. Miles ; Alistair L. Wilkins ; John S. Munday ; Rex Munday ; Allan D. Hawkes ; Dwayne J. Jensen ; Janine M. Cooney ; and Veronica Beuzenberg
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2006
• 出版时间：February 22, 2006
• 年：2006
• 卷：54
• 期：4
• 页码：1530 - 1534
• 全文大小：55K
• 年卷期：v.54,no.4(February 22, 2006)
• ISSN：1520-5118

文摘

Pectenotoxins (PTXs) accumulate in shellfish feeding on dinoflagellates of the genus Dinophysis, sothat humans can be exposed to these toxins through shellfish consumption. Some PTXs are toxic toexperimental animals, whereas others are of much lower toxicity. Pectenotoxin-2, the most abundantPTX from most Dinophysis spp., is rapidly metabolized by most shellfish to a mixture of pectenotoxin-2seco acid (2) and 7-epi-pectenotoxin-2 seco acid (1). A mixture of 1 and 2 was produced duringpurification of an extract from in vitro enzymatic hydrolysis of pectenotoxin-2. These were separatedby preparative HPLC, and the structure of 1 was confirmed by one- and two-dimensional ¹H and ¹³CNMR spectroscopy and LC-MS³ analyses. No toxic changes were recorded in mice injectedintraperitoneally with 1 or 2 at a dose of 5000 g/kg. PTX seco acids are therefore unlikely to be ofconsequence to human consumers at the concentrations found in contaminated shellfish.Keywords: pectenotoxin; seco acid; PTX-2 seco acid; Dinophysis; toxicology; mussel