Visualization of Multimerization and Self-Assembly of DNA-Functionalized Gold Nanoparticles Using In-Liquid Transmission Electron Microscopy

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• 作者：Sercan Keskin ; Stephanie Besztejan ; G眉nther Kassier ; Stephanie Manz ; Robert B眉cker ; Svenja Riekeberg ; Hoc Khiem Trieu ; Andrea Rentmeister ; R. J. Dwayne Miller
• 刊名：The Journal of Physical Chemistry Letters
• 出版年：2015
• 出版时间：November 19, 2015
• 年：2015
• 卷：6
• 期：22
• 页码：4487-4492
• 全文大小：367K
• ISSN：1948-7185

文摘

Base-pairing stability in DNA-gold nanoparticle (DNA-AuNP) multimers along with their dynamics under different electron beam intensities was investigated with in-liquid transmission electron microscopy (in-liquid TEM). Multimer formation was triggered by hybridization of DNA oligonucleotides to another DNA strand (Hyb-DNA) related to the concept of DNA origami. We analyzed the degree of multimer formation for a number of samples and a series of control samples to determine the specificity of the multimerization during the TEM imaging. DNA-AuNPs with Hyb-DNA showed an interactive motion and assembly into 1D structures once the electron beam intensity exceeds a threshold value. This behavior was in contrast with control studies with noncomplementary DNA linkers where statistically significantly reduced multimerization was observed and for suspensions of citrate-stabilized AuNPs without DNA, where we did not observe any significant motion or aggregation. These findings indicate that DNA base-pairing interactions are the driving force for multimerization and suggest a high stability of the DNA base pairing even under electron exposure.