Translocation of Poly(ethylene glycol-co-hexadecyl)cyanoacrylate Nanoparticles into Rat Brain Endothelial Cells: Role of Apolipoproteins in Receptor-Mediated Endocytosis

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• 作者：Hyun R Kim ; Karine Andrieux ; Sophie Gil ; Myriam Taverna ; H&eacute ; l&eacute ; ne Chacun ; Didier Desmaë ; le ; Fr&eacute ; deric Taran ; Dominique Georgin ; Patrick Couvreur
• 刊名：Biomacromolecules
• 出版年：2007
• 出版时间：March 2007
• 年：2007
• 卷：8
• 期：3
• 页码：793 - 799
• 全文大小：336K
• 年卷期：v.8,no.3(March 2007)
• ISSN：1526-4602

文摘

Previous in vivo observations in rats have shown that poly(ethylene glycol) polyhexadecylcyanoacrylate (PEG-PHDCA) nanoparticles could translocate into the brain after intravenous injection, which polyhexadecylcyanoacrylate (PHDCA) nanoparticles did not. Through the detailed analysis of the plasma protein adsorption ontothe surface of PEG-PHDCA nanoparticles, the present study aimed at clarifying the mechanism by whichnanoparticles could penetrate into rat brain endothelial cells (RBEC). Two-dimensional polyacrylamide gelelectrophoresis and Western blotting revealed that, after incubation with rat serum, apolipoprotein E (ApoE)adsorbed more onto PEG-PHDCA than on PHDCA nanoparticles. Adsorption of apolipoprotein B-100 (ApoB-100) onto PEG-PHDCA nanoparticles was demonstrated by capillary electrophoresis experiments. Moreover,only when ApoE or ApoB-100 were preadsorbed onto PEG-PHDCA nanoparticles, nanoparticles were found tobe more efficient than control nanoparticles for penetrating into RBEC, suggesting the involvement of a lowdensity lipoprotein receptor in this process. Thus, these data clearly demonstrate the involvement of apolipoproteinsin the brain transport of PEG-PHDCA nanoparticles, which may open interesting prospects for brain drug delivery.