Antibodies to Citrullinated Human Fibrinogen Synthetic Peptides in Diagnosing Rheumatoid Arthritis
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- 作者：Marí ; a L. Pé ; rez ; Marí ; a J. Gó ; mara ; Guadalupe Ercilla ; Raimon Sanmartí ; Isabel Haro
- 刊名：Journal of Medicinal Chemistry
- 出版年：2007
- 出版时间：July 26, 2007
- 年：2007
- 卷：50
- 期：15
- 页码：3573 - 3584
- 全文大小：493K
- 年卷期：v.50,no.15(July 26, 2007)
- ISSN：1520-4804
文摘
Since aggressive therapy given early in the rheumatoid arthritis (RA) disease course has the greatest therapeuticpotential, early diagnostic tests with both high specificity and sensitivity are desirable. Rheumatoid serawere found to contain antibodies against citrullinated peptides, which are considered to be highly specificmarkers of RA. In the present work several analogues of the 
- and 
-chains of fibrin peptides containingdifferent degrees of citrullination have been synthesized and analyzed by ELISA using 111 sera from RApatients. In addition, we have also investigated the synergistic effects of different presentation formats ofthe synthetic constructs. We have designed chimeric and cyclic peptides that bear different peptide sequenceswithin the same molecule. Our results indicate that the synthesis of peptides bearing fibrinogen and filaggrindomains could be a robust method for the design of useful diagnostic strategies in RA.
- and -chains of fibrin peptides containingdifferent degrees of citrullination have been synthesized and analyzed by ELISA using 111 sera from RApatients. In addition, we have also investigated the synergistic effects of different presentation formats ofthe synthetic constructs. We have designed chimeric and cyclic peptides that bear different peptide sequenceswithin the same molecule. Our results indicate that the synthesis of peptides bearing fibrinogen and filaggrindomains could be a robust method for the design of useful diagnostic strategies in RA.