Layer-by-Layer Construction of Ultrathin Hybrid Films with Proteins and Clay Minerals

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• 作者：Tamá ; s Szabó ; Má ; rta Szekeres ; Imre D&eacute ; ká ; ny ; Carine Jackers ; Steven De Feyter ; Cliff T. Johnston ; Robert A. Schoonheydt
• 刊名：Journal of Physical Chemistry C
• 出版年：2007
• 出版时间：August 30, 2007
• 年：2007
• 卷：111
• 期：34
• 页码：12730 - 12740
• 全文大小：832K
• 年卷期：v.111,no.34(August 30, 2007)
• ISSN：1932-7455

文摘

Mono- and multilayered clay mineral-protein films were constructed with the layer-by-layer depositiontechnique. The clay mineral saponite and three proteins (protamine, lysozyme, and papain) were tested.Multilayers with up to 15-15 alternating layers of saponite and protein were built up on glass, quartz, andZnSe. In some cases these supports were primed with the poly(diallyldimethylammonium) cation before clay/protein deposition. The deposition process can be followed by UV and attenuated total reflectance Fouriertransform infrared (ATR-FTIR) spectroscopy and by atomic force microscopy (AFM). X-ray diffraction (XRD)patterns confirm the regular ordering of the layers. The "average" protein layer has the thickness of singlemolecules. The thickness of the "average" saponite layer is proportional to the positive charge density of theprotein and varies between 0.6 and 3 elementary saponite sheets. The layers of protamine and lysozyme arestable. However, a small amount of papain is lost upon deposition of the subsequent saponite layer. H-Dexchange of proteins in the films is fast and reversible.