Beta vulgaris L. Suspension Cultures Permeabilized with Triton X-100 Retain Cell Viability and Betacyanines Production Ability: A Digital Image Analysis Study
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- 作者：Gabriela Trejo-Tapia ; Jonathan Cuevas-Celis ; Guadalupe Salcedo-Morales ; José ; Luis Trejo-Espino ; Martha L. Arenas-Ocampo ; Antonio Jimé ; nez-Aparicio
- 刊名：Biotechnology Progress
- 出版年：2007
- 出版时间：April 2007
- 年：2007
- 卷：23
- 期：2
- 页码：359 - 363
- 全文大小：123K
- 年卷期：v.23,no.2(April 2007)
- ISSN：1520-6033
文摘
The influence of Triton X-100 on Beta vulgaris L. permeabilized cell culture viability, regrowth,and ability to produce betacyanines was evaluated in this study. A non-destructive method basedon the analysis of images in the RGB (red, green, blue) system was developed to estimatebetacyanines content. A treatment for 15 min with 0.7 mM Triton X-100 induced the release of30% of betacyanines without loss of cell viability (
70%). After this permeabilization treatment,B. vulgaris cultures regrew normally, reaching a maximum biomass concentration of 48% higherthan non-permeabilized cultures after 14 days of culture. Also, maximum betacyaninesconcentration was only 25% lower than that of non-permeabilized cultures.
70%). After this permeabilization treatment,B. vulgaris cultures regrew normally, reaching a maximum biomass concentration of 48% higherthan non-permeabilized cultures after 14 days of culture. Also, maximum betacyaninesconcentration was only 25% lower than that of non-permeabilized cultures.