Quantitative and Confirmatory Analyses of Malachite Green and Leucomalachite Green Residues in Fish and Shrimp

详细信息    查看全文

• 作者：Wendy C. Andersen ; Sherri B. Turnipseed ; and Jos&eacute ; E. Roybal
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2006
• 出版时间：June 28, 2006
• 年：2006
• 卷：54
• 期：13
• 页码：4517 - 4523
• 全文大小：264K
• 年卷期：v.54,no.13(June 28, 2006)
• ISSN：1520-5118

文摘

Liquid chromatographic methods are presented for the quantitative and confirmatory determinationof malachite green (MG) and leucomalachite green (LMG) for channel catfish, rainbow trout, tilapia,basa, Atlantic salmon, and tiger shrimp. Residues were extracted from tissues with ammonium acetatebuffer and acetonitrile and isolated by partitioning into dichloromethane. LMG was quantitativelyoxidized to the chromic MG with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone. Extracts were analyzedfor total MG by liquid chromatography with both visible detection (LC-VIS) at 618 nm for routinescreening and ion trap mass spectrometry (LC-MSⁿ) with no discharge-atmospheric pressure chemicalionization for residue confirmation. The method was validated in each species fortified with LMG at1, 2, 4, and 10 ng/g (ppb), and average recoveries ranged from 85.9 to 93.9%. Quantitative datawere consistent for the two detection methods, with measured method detection limits of 1.0 ng/g forLC-VIS and 0.25 ng/g for LC-MSⁿ. Incurred tissues from catfish, trout, tilapia, and salmon that hadbeen treated with MG were also extracted and analyzed as part of this study.Keywords: Malachite green; leucomalachite green; LC-VIS; LC-MSⁿ; ND-APCI; fish; shrimp