FT-IR Investigation of Cell Wall Polysaccharides from Cereal Grains. Arabinoxylan Infrared Assignment
详细信息 查看全文
- 作者：Paul Robert ; Mé ; lanie Marquis ; Cé ; cile Barron ; Fabienne Guillon ; and Luc Saulnier
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2005
- 出版时间：September 7, 2005
- 年：2005
- 卷：53
- 期：18
- 页码：7014 - 7018
- 全文大小：81K
- 年卷期：v.53,no.18(September 7, 2005)
- ISSN：1520-5118
文摘
The FT-IR fingerprint of wheat endosperm arabinoxylan (AX) was investigated using a set ofpolysaccharides exhibiting variation of their degree of substitution and xylo-oligosaccharides comprisingxylose units mono- or disubstituted by arabinose residues. Substitution of the xylose backbone byarabinose side units was more particularly studied in the 1000-800 cm-1 spectral region, by takingadvantage of second-derivative enhancement. The 920-1020 cm-1 spectral region revealed twoabsorption bands at 984 and 958 cm-1, the intensities of which varied according to the degree ofsubstitution. Whereas the intensity of the band at 958 cm-1 increased with the degree of substitution,that at 984 cm-1 decreased. The second-derivative spectral data of xylo-oligosaccharides indicatedthat these changes could be attributed to substitution of the xylan backbone by arabinose residues,and the band at 958 cm-1 was ascribed to the presence of disubstituted xylose residues. Principalcomponent analysis of FT-IR spectra of model mixtures of AX, 
ges/gifchars/beta2.gif" BORDER=0 ALIGN="middle">-glucans, and arabinogalactanssuggested that it is possible to evaluate the relative proportions of the polymers and degree ofsubstitution of AX in complex mixtures such as the cell wall of cereal grains.Keywords: Hemicellulose; ges/gifchars/beta2.gif" BORDER=0 ALIGN="middle">-glucan; arabinogalactan; pentosan; wheat; principal component analysis