NMR and HPLC-UV Profiling of Potatoes with Genetic Modifications to Metabolic Pathways

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• 作者：Marianne Defernez ; Yvonne M. Gunning ; Adrian J. Parr ; Louise V. T. Shepherd ; Howard V. Davies ; and Ian J. Colquhoun
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2004
• 出版时间：October 6, 2004
• 年：2004
• 卷：52
• 期：20
• 页码：6075 - 6085
• 全文大小：193K
• 年卷期：v.52,no.20(October 6, 2004)
• ISSN：1520-5118

文摘

Metabolite profiling has been carried out to assess the compositional changes occurring in potatotubers after genetic modifications have been made to different metabolic pathways. Most majorfeatures in the ¹H NMR and HPLC-UV profiles of tuber extracts have been assigned. About 40 GMlines and controls belonging to 4 groups of samples (derived from cv. Record or cv. Desirée andmodified in primary carbon metabolism, starch synthesis, glycoprotein processing, or polyamine/ethylene metabolism) were analyzed. Differences were assessed at the level of whole profiles (byPCA) or individual compounds (by ANOVA). The most obvious differences seen in both NMR andHPLC-UV profiles were between the two varieties. There were also significant differences betweentwo of the four Desirée GM lines with modified polyamine metabolism and their controls. Compoundsnotably affected were proline, trigonelline, and numerous phenolics. However, that modification gaverise to a very abnormal phenotype. Certain lines from the other groups had several compounds presentin significantly higher or lower amounts compared to the control, but the differences in mean valuesamounted to no more than a 2-3-fold change: in the context of variability in the whole data set,such changes did not appear to be important.Keywords: Solanum tuberosum L.; potato; metabolite profiling; metabolomics; NMR; HPLC; chemometrics; multivariate; transgenic; GMO