Cryo-Fracture TEM: Direct Imaging of a Random Mesh Phase

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• 作者：Sanja Bulut ; Alfredo Gonzá ; lez-P&eacute ; rez ; Ulf Olsson
• 刊名：Langmuir
• 出版年：2008
• 出版时间：January 1, 2008
• 年：2008
• 卷：24
• 期：1
• 页码：22 - 25
• 全文大小：527K
• 年卷期：v.24,no.1(January 1, 2008)
• ISSN：1520-5827

文摘

A novel and a simple method that allows direct imaging of viscous samples by cryo-TEM (cryo-transmissionelectron microscopy) is presented. A fracture on the vitrified sample is created in a controlled way. In the fracture,some edges are thin enough to allow direct imaging in transmission mode. The method was used to directly imagea nonionic surfactant lamellar phase where a random mesh structure is formed at lower temperatures (<10 C). Aso-called random mesh phase, characterized by the presence of perforated surfactant bilayers, is imaged here for thefirst time. Images from the mesh structure are compared with images from the classical lamellar structure formed atroom temperature.