Fluorescent II-VI Semiconductor Quantum Dots in Living Cells: Nonlinear Microspectroscopy in an Optical Tweezers System

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• 作者：Patricia M. A. Farias ; Beate S. Santos ; Andr&eacute ; A. de Thomaz ; Ricardo Ferreira ; Frederico D. Menezes ; Carlos L. Cesar ; Adriana Fontes
• 刊名：Journal of Physical Chemistry B
• 出版年：2008
• 出版时间：March 6, 2008
• 年：2008
• 卷：112
• 期：9
• 页码：2734 - 2737
• 全文大小：233K
• 年卷期：v.112,no.9(March 6, 2008)
• ISSN：1520-5207

文摘

In this work we used a setup consisting of an optical tweezers combined with a nonlinear microspectroscopysystem to perform scanning microscopy and obtain emission spectra using two photon excited (TPE)luminescence of captured single living cells labeled with core-shell fluorescent semiconductor quantum dots(QDs). The QDs were obtained via colloidal synthesis in aqueous medium with an adequate physiologicalresulting pH. Sodium polyphosphate was used as the stabilizing agent. The results obtained show the potentialpresented by this system as well as by these II-VI fluorescent semiconductor quantum dots to performspectroscopy in living trapped cells in any neighborhood and dynamically observe the cell chemical reactionsin real time.