An Investigation on the Analytical Potential of Polymerized Liposomes Bound to Lanthanide Ions for Protein Analysis

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• 作者：Marina Santos ; Bidhan C. Roy ; H&eacute ; ctor Goicoechea ; Andres D. Campiglia ; and Sanku Mallik
• 刊名：Journal of the American Chemical Society
• 出版年：2004
• 出版时间：September 1, 2004
• 年：2004
• 卷：126
• 期：34
• 页码：10738 - 10745
• 全文大小：156K
• 年卷期：v.126,no.34(September 1, 2004)
• ISSN：1520-5126

文摘

We present a promising approach to protein sensing based on Eu³⁺ ions incorporated intopolymerized liposomes. The sensitization of Eu³⁺ is accomplished with 5-aminosalicylic acid, which providesenergy transfer for a stable reference signal and a wide wavelength excitation range free from proteininterference. The lipophilic character of polymerized liposomes provides the appropriate platform for proteininteraction with the lanthanide ion. Quantitative analysis is based on the linear relationship between theluminescence signal of Eu³⁺ and protein concentration. Because no spectral shift of the lanthanideluminescence is observed upon protein interaction, qualitative analysis is based on the luminescence lifetimeof polymerized liposomes. This parameter, which changes significantly upon protein-liposome interaction,follows a well-behaved single-exponential decay that might be useful for protein identification.