Microfluidic Device for Single-Molecule Experiments with Enhanced Photostability
详细信息 查看全文
- 作者：Edward A. Lemke ; Yann Gambin ; Virginia Vandelinder ; Eric M. Brustad ; Hsiao-Wei Liu ; Peter G. Schultz ; Alex Groisman ; Ashok A. Deniz
- 刊名：Journal of the American Chemical Society
- 出版年：2009
- 出版时间：September 30, 2009
- 年：2009
- 卷：131
- 期：38
- 页码：13610-13612
- 全文大小：201K
- 年卷期：v.131,no.38(September 30, 2009)
- ISSN：1520-5126
文摘
A microfluidic device made of polydimethylsiloxane (PDMS) addresses key limitations in single-molecule fluorescence experiments by providing high dye photostability and low sample sticking. Photobleaching is dramatically reduced by deoxygenation via gas diffusion through porous channel walls. Rapid buffer exchange in a laminar sheath flow followed by optical interrogation minimizes surface−sample contacts and allows the in situ addition and combination of other reagents.