Single Cell Tracking of Gadolinium Labeled CD4+ T Cells by Laser Ablation Inductively Coupled Plasma Mass Spectrometry

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• 作者：Amy J. Managh ; Sheldon L. Edwards ; Andrew Bushell ; Kathryn J. Wood ; Edward K. Geissler ; James A. Hutchinson ; Robert W. Hutchinson ; Helen J. Reid ; Barry L. Sharp
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：November 19, 2013
• 年：2013
• 卷：85
• 期：22
• 页码：10627-10634
• 全文大小：349K
• 年卷期：v.85,no.22(November 19, 2013)
• ISSN：1520-6882

文摘

Cellular therapy is emerging as a promising alternative to conventional immunosuppression in the fields of hematopoietic stem cell (HSC) transplantation, autoimmune disease, and solid organ transplantation. Determining the persistence of cell-based therapies in vivo is crucial to understanding their regulatory function and requires the combination of an extremely sensitive detection technique and a stable, long-lifetime cell labeling agent. This paper reports the first application of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to perform single cell detection of T cell populations relevant to cellular immunotherapy. Purified human CD4⁺ T cells were labeled with commercially available Gd-based magnetic resonance imaging (MRI) contrast agents, Omniscan and Dotarem, which enabled passive loading of up to 10⁸ Gd atoms per cell. In mixed preparations of labeled and unlabeled cells, LA-ICP-MS was capable of enumerating labeled cells at close to the predicted ratio. More importantly, LA-ICP-MS single cell analysis demonstrated that the cells retained a sufficient label to remain detectable for up to 10 days post-labeling both in vitro and in vivo in an immunodeficient mouse model.