Use of Soybean Peroxidase in Chemiluminescent Enzyme-Linked Immunosorbent Assay

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• 作者：Ivan Yu. Sakharov ; Inna S. Alpeeva ; and Evgeny E. Efremov
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2006
• 出版时间：March 8, 2006
• 年：2006
• 卷：54
• 期：5
• 页码：1584 - 1587
• 全文大小：61K
• 年卷期：v.54,no.5(March 8, 2006)
• ISSN：1520-5118

文摘

A procedure for the production of conjugates of soybean peroxidase (SbP) oxidized by sodiumperiodate and anti-mouse IgG antibody (Ab) was optimized. A sandwich chemiluminescent enzyme-linked immunosorbent assay (ELISA) for determination of mouse IgG using SbP and specific Abwas developed, and SbP-catalyzed oxidation of luminol was carried out in the absence of anyenhancer. Comparison of conjugates produced by labeling Ab by soybean and horseradishperoxidases in the chemiluminescent ELISA showed that in the case of SbP a rate of emission decayformed through luminol oxidation was significantly lower. Application of the soya enzyme allowedthe development of the immunoassay with improved sensitivity and a wider linear range.Keywords: Peroxidase; soya; ELISA; chemiluminescence; luminol; hydrogen peroxide; enhancement