Side-Chain Structure of Cell Surface Polysaccharide, Mannan, Affects Hypocholesterolemic Activity of Yeast

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• 作者：Yasuto Yoshida ; Eiichiro Naito ; Harumi Mizukoshi ; Yoko Watanabe ; Kazumasa Kimura ; Wakae Yokoi ; Tadashi Sato ; Takekazu Okumura ; Masahiko Ito ; Haruji Sawada
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2009
• 出版时间：September 9, 2009
• 年：2009
• 卷：57
• 期：17
• 页码：8003-8009
• 全文大小：842K
• 年卷期：v.57,no.17(September 9, 2009)
• ISSN：1520-5118

文摘

We previously reported that Kluyveromyces marxianus YIT 8292 exhibited more potent hypocholesterolemic activity than other yeasts containing Saccharomyces cerevisiae. To clarify the reason for the higher hypocholesterolemic activity, we examined the side-chain structure of cell surface polysaccharide, mannan, of K. marxianus YIT 8292. The result shows that K. marxianus YIT 8292 had shorter α-(1,2)-linked oligomannosyl side chains and lower phosphate content in mannan than S. cerevisiae. The association between its structural features and hypocholesterolemic activity was investigated by comparing the hypocholesterolemic activities of S. cerevisiae mannan mutants in rats fed a high-cholesterol diet. S. cerevisiae mnn5 mutant with deficiencies in the phosphorylation and elongation of mannan side chains showed higher hypocholesterolemic activity than the wild-type strain. These results show that the side-chain length and phosphate contents of mannan affect hypocholesterolemic activity.