A Luminescence Sensor of Inositol 1,4,5-Triphosphate and Its Model Compound by Ruthenium-Templated Assembly of a Bis(Zn2+-Cyclen) Complex Having a 2,2'-Bipyridyl Linker (Cyclen = 1,4,7,10-T
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- 作者：Shin Aoki ; Mohd Zulkefeli ; Motoo Shiro ; Masanori Kohsako ; Kei Takeda ; and Eiichi Kimura
- 刊名：Journal of the American Chemical Society
- 出版年：2005
- 出版时间：June 29, 2005
- 年：2005
- 卷：127
- 期：25
- 页码：9129 - 9139
- 全文大小：599K
- 年卷期：v.127,no.25(June 29, 2005)
- ISSN：1520-5126
文摘
A new supramolecular complex (Ru(Zn2L4)3) was designed and synthesized as a luminescencesensor for inositol 1,4,5-triphosphate (IP3), which is one of the important second messengers in intracellularsignal transduction, and its achiral model compound, cis,cis-1,3,5-cyclohexanetriol triphosphate (CTP3),by a ruthenium(II)-templated assembly of three molecules of a bis(Zn2+-cyclen) complex having a 2,2-bipyridyl linker (Zn2L4). Single-crystal X-ray diffraction analysis of a racemic mixture of Ru(Zn2L4)3 showedthat three of the six Zn2+-cyclen units are orientated to face the opposite side of the molecule with threeapical ligands (Zn2+-bound HO-) of each of the three Zn2+ located on the same face. 1H NMR and UVtitrations of Ru(Zn2L4)3 with CTP3 indicated that Ru(Zn2L4)3 forms a 1:2 complex with CTP3, (Ru(Zn2L4)3)-((CTP3)6-)2, in aqueous solution at neutral pH. In the absence of guest molecules, Ru(Zn2L4)3 (10 
M) hasan emission maximum at 610 nm at pH 7.4 (10 mM HEPES with I = 0.1 (NaNO3)) and 25 
C (excitationat 300 nm). An addition of 2 equiv of CTP3 induced a 4.2-fold enhancement in the emission of Ru(Zn2L4)3at 584 nm. In this article, we describe that Ru(Zn2L4)3 is the first chemical sensor that directly responds toCTP3 and IP3 and discriminates these triphosphates from monophosphates and diphosphates. Thephotodecomposition of Ru(Zn2L4)3, which is inhibited upon complexation with CTP3, and the stereoselectivecomplexation of chiral IP3 by Ru(Zn2L4)3 are also described.
M) hasan emission maximum at 610 nm at pH 7.4 (10 mM HEPES with I = 0.1 (NaNO3)) and 25 C (excitationat 300 nm). An addition of 2 equiv of CTP3 induced a 4.2-fold enhancement in the emission of Ru(Zn2L4)3at 584 nm. In this article, we describe that Ru(Zn2L4)3 is the first chemical sensor that directly responds toCTP3 and IP3 and discriminates these triphosphates from monophosphates and diphosphates. Thephotodecomposition of Ru(Zn2L4)3, which is inhibited upon complexation with CTP3, and the stereoselectivecomplexation of chiral IP3 by Ru(Zn2L4)3 are also described.