First Signal from a Broadband Cryogenic Preamplifier Cooled by Circulating Liquid Nitrogen in a 7 T Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

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• 作者：Myoung Choul Choi ; Jeong Min Lee ; Se Gyu Lee ; Sang Hwan Choi ; Yeon Suk Choi ; Kyung Jae Lee ; SeungYong Kim ; Hyun Sik Kim ; Stefan Stahl
• 刊名：Analytical Chemistry
• 出版年：2012
• 出版时间：December 18, 2012
• 年：2012
• 卷：84
• 期：24
• 页码：10543-10548
• 全文大小：419K
• 年卷期：v.84,no.24(December 18, 2012)
• ISSN：1520-6882

文摘

Despite the outstanding performance of Fourier transform ion cyclotron/mass spectrometry (FTICR/MS), the complexity of the cellular proteome or natural compounds presents considerable challenges. Sensitivity is a key performance parameter of a FTICR mass spectrometer. By improving this parameter, the dynamic range of the instrument can be increased to improve the detection signal of low-abundance compounds or fragment ion peaks. In order to improve sensitivity, a cryogenic detection system was developed by the KBSI (Korean Basic Science Institute) in collaboration with Stahl-Electronics (Mettenheim, Germany). A simple, efficient liquid circulation cooling system was designed and a cryogenic preamplifier implemented inside a FTICR mass spectrometer. This cooling system circulates a cryoliquid from a Dewar to the 鈥渓iquid circulation unit鈥?through a CF flange to cool a copper block and a cryopreamplifier; the cooling medium is subsequently exhausted into the air. The cryopreamplifier can be operated over a very wide temperature range, from room temperature to low temperature environments (4.2 K). First, ion signals detected by the cryopreamplifier using a circulating liquid nitrogen cooling system were observed and showed a signal-to-noise ratio (S/N) about 130% better than that obtained at room temperature.