Determination of the Gelation Mechanism of Freeze鈥揟hawed Hen Egg Yolk

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• 作者：Carmen Au ; Nuria C. Acevedo ; Harry T. Horner ; Tong Wang
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：November 25, 2015
• 年：2015
• 卷：63
• 期：46
• 页码：10170-10180
• 全文大小：575K
• ISSN：1520-5118

文摘

A study of yolks stored up to 168 d at 鈭?0 掳C was conducted to determine the gelation behavior and mechanism of freeze鈥搕hawed yolk. Methods used were rheology, native and sodium dodecyl sulfate polyacrylamide gel electrophoresis (native- and SDS-PAGE), differential scanning calorimetry (DSC), transmission electron microscopy (TEM), particle size analysis, and proton nuclear magnetic resonance (¹H NMR) spectroscopy for matrix mobility. Results indicate that both constituents of plasma and granules contributed to gelation of yolk under freezing. PAGE analyses suggest that granular proteins participated in aggregation during freeze鈥搕haw. Increasing gel strength and particle size and decreasing water and lipid鈥搘ater mobility indicate that lipoproteins or apolipoproteins aggregated. At storage times 鈮?4 d, increased protein and lipid mobility, the detection of smaller particles, and secondarily increased gel strength suggest the liberation of protein or lipoprotein components from previously formed aggregates and further aggregation of these constituents. Disruption of the gelled yolk matrix observed with TEM supported that ice crystal formation was required for gelation to occur. A two-stage dynamic gelation model is thus proposed.